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Fixing DNA breaks during class switch recombination.

Immunoglobulin (Ig) class switch recombination (CSR) involves the breakage and subsequent repair of two DNAsequences, known as switch (S) regions, which flank IgH constant region exons. The resolution of CSR-associated breaks is thought to require the nonhomologous end-joining (NHEJ) DNA repair pathway, but the role of the NHEJ factor DNA-dependent protein kinase catalytic subunit (DNA-PKcs) in this process has been unclear. A new study, in which broken IgH-containing chromosomes in switching B cells were visualized directly, clearly demonstrated that DNA-PKcs and, unexpectedly, the nuclease Artemis are involved in the resolution of switchbreaks.

Jolly, C., Cook, A., Manis, J.

The Journal of Experimental Medicine, 205(3), 509-513.

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