Home > Publications > Proximity to AGCT sequences dictates MMR-independent versus MMR-dependent mechanisms for AID-induced mutation via UNG2

Proximity to AGCT sequences dictates MMR-independent versus MMR-dependent mechanisms for AID-induced mutation via UNG2

Abstract AID deaminates C to U in either strand of Ig genes, exclusively producing C: G/G: C
to T: A/A: T transition mutations if U is left unrepaired. Error-prone processing by UNG2 or
mismatch repair diversifies mutation, predominantly at C: G or A: T base pairs, respectively.
Here, we show that transversions at C: G base pairs occur by two distinct processing
pathways that are dictated by sequence context. Within and near AGCT mutation hotspots,
transversion mutation at C: G was driven by UNG2 without requirement for mismatch …

Eddy Sanchai Thientosapol, George Sharbeen, KK Lau, Daniel Bosnjak, Timothy Durack, Igor Stevanovski, Wolfgang Weninger, Christopher J Jolly

Nucleic Acids Research

Date: 2017

Volume 45 | Issue 6