Dr Sriharsa Pradhan
Distinguished Scientist, Genome Biology Division
New England Biolabs, Inc
Mammalian chromatin is dynamic, and contains DNA regulatory elements that lead to it’s accessibility to transcription factors, and gene expression. We developed a novel accessible chromatin mapping methodology using Nicking Enzyme assisted sequencing (NicE-seq) for integrative epigenomic analysis. Universal NicE-seq captures accessible chromatin sites (ACS) in cells and reveals their genomic location and transcription factor occupancy at bp resolution, with ~ 250 kidney cells. ACS correlated with RNA pol II occupancy and transcriptionally active chromatin marks, while displaying a pattern contrasting to CpG methylation. Differential ACS were observed in human fetal vs. adult tissue sections.
Dr Pradhan obtained a PhD at Glasgow University, Biochemistry and Molecular Biology in 1995, completed 4 years Post-Doc with Nobel Laurate Sir Richard J. Roberts and currently holds the position of adjunct Prof at the Biology Dept, Boston University. Dr Pradhan started his scientific career by studying epigenetics enzymes characterization and mechanism, shifting his attention to mammalian DNA modifications and epigenome characterization in 2006. His research focuses on development of new technologies to answer basic biological questions. Chromatin structure, modifications and organizations determines the cell fate and his work focuses on accessible chromatin structure and alterations during cell development and diseases, particularly cancer and tumor heterogeneity.