The relationship between the organisation of the genome, over both space and time, and its function, is an outstanding question in biology. This key biological process faces dysregulation and underpins many diseases such as cancer and cardio-vascular disorders.
The establishment of the Centenary Institute’s Genome Imaging Centre and its advanced new super resolution microscopy allows for high-contrast single molecule imaging in living cells in order to decipher the molecular principle of gene regulation dynamics.
Offering a comprehensive technology training platform, the Centre is able to undertake quantitative molecular imaging of the dynamic genome with a freely accessible repository and analysis toolkit.
This rare specialised training and multi-modal imaging capability enhances opportunities to extend and enrich scientific knowledge across Centenary’s existing medical research programs, with new external users also supported.
The Genome Imaging Centre is the first of its kind in Australia, providing new capability in basic science to analyse and better understand molecular mechanisms in gene regulation. Applied science–including innovative drug discovery, industry collaboration and potential future biotechnology spin-outs is also a focus.
Technology training by the Centre provides non-expert research groups with access to cutting edge new approaches in quantitative molecular imaging applied to genome biology.
The Centre is able to assist in experimental design and training applied to single molecule localisation microscopy such as TIRFF, HILO, dSTORM and STEAD. Access to a unique computational pipeline to process, analyse and visualise large datasets is also provided. A transcription factor dashboard developed in collaboration with the Sydney Informatics Hub – The University of Sydney serves as a repository for single molecule data sets that could be used for data such as chromatin mobility and epigenetics maps.
The Centre supports the development of new methods applied to genome imaging such as PAINT, smFISH, MERFISH or LIVE MIEL based on unique access to this technology via a national and international network of collaborators across the globe.
Associate Professor Till Boecking, UNSW
Professor Susan Clark, Garvan Institute
Dr Matt Graus, Centenary Institute
Dr Elizabeth Hinde, University of Melbourne
Dr Peter Su, University of Technology Sydney
Distinguished Professor Antoine van Oijen, University of Wollongong
Dr Emily Wong, Victor Chang Cardiac Research Institute
Peter MacCallum Cancer Centre, Kieran Harvey laboratory
University of Melbourne, Elizabeth Hinde laboratory
University of Sydney, Charles Perkins Centre
University of Sydney, Sydney Informatic Hub (SIH)
University of Technology Sydney, Peter Su laboratory
Victor Chang Cardiac Research Institute, Richard Harvey laboratory
Hiroshima University, Hiroshi Ochiai laboratory
IRCCS Opedale San Raffaele, Davide Mazza laboratory
Sanford Burnham Prebys Medical Discovery Institute, Alexey Tserkikh laboratory
University of Ulm, Christof Gebhardt laboratory